The smart Trick of PP88 That No One is Discussing
The smart Trick of PP88 That No One is Discussing
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In a selected embodiment, stated ex vivo strategy is for protecting a floor in opposition to biofouling. In Yet another individual embodiment, stated ex vivo technique is for decontaminating drinking water.
The sequence encoding Mad4 (the whole protein or a fraction thereof) as Utilized in the context of the creation is disclosed in international software WO2018/236548.
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18. the strategy In keeping with embodiment 17, wherein stated conditional origin of replication is active in reported donor bacterial cell because said donor bacterial mobile expresses a rep protein, especially a primase-helicase.
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In a particular embodiment, mentioned conditional origin of replication may be the primase ori from the PICI from the Escherichia coli strain CFT073, devoid on the restriction site GAAABCC. Preferably, stated conditional origin of replication is of sequence SEQ ID NO: 6.
FIG. eleven: Nuclease-mediated killing of four O157 strains mediated by stx targeting after transduction of packaged phagemids harboring a conditional origin of replication (payload p1327).
wherein explained vector additional comprises a conditional origin of replication that is inactive inside the targeted receiver bacterial cell but is Energetic in a donor bacterial mobile, and claimed vector is devoid of antibiotic resistance marker,
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11. Pragmatic Engage in reserves the proper to amend, suspend or terminate the marketing at any time and without the need of prior recognize.
In a certain embodiment, explained donor bacterial cell can be a production cell line, particularly a cell line producing packaged phagemids including the vector of the creation.
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